Welcome to Human IRES Atlas

(Sample Genes: FGF9, SLC7A1, RUNX1)

Introduction

It is now known that cap-independent translation initiation facilitated by internal ribosome entry sites (IRES) is vital in selective cellular protein synthesis under stress and different physiological conditions. However, three problems make it hard to understand transcriptome-wide cellular IRES-mediated translation initiation mechanisms: (1) complex interplay between IRESs and other translation initiation-related information; (2) reliability issue of in silico cellular IRES investigation; (3) labor-intensive in vivo IRES identification. In this research, we constructed the Human IRES Atlas database for a comprehensive understanding of cellular IRES elements in humans. First, currently available and suitable IRES prediction tools (IRESfinder, PatSearch and IRESpy) were used to obtain transcriptome-wide human IRES elements. Then we collected eight genres of translation initiation-related features to help study the potential molecular mechanisms of each of the putative IRES elements. Three functional tests (conservation, RNA-protein score and conditional translation efficiency) were devised to help evaluate the identified putative IRES elements' functionality. Moreover, an easy-to-use interface and an IRES-translation initiation interaction map for each gene transcript were implemented to help understand the interactions between IRESs and translation initiation-related features. Researchers can easily search/browse an IRES of interest using the web-interface and deduce testable mechanism hypotheses of human IRES-driven translation initiation based on the integrated results.

History

2021/03 -- Revised database released !
2021/02 -- Data and web-interface updated !
2020/11 -- Human IRES Atlas released !